disk confocal microscopy Search Results


spin sr spinning disk confocal microscope system  (Evident Corporation)
96
Evident Corporation spin sr spinning disk confocal microscope system
Spin Sr Spinning Disk Confocal Microscope System, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spin sr spinning disk confocal microscope system/product/Evident Corporation
Average 96 stars, based on 1 article reviews
spin sr spinning disk confocal microscope system - by Bioz Stars, 2026-03
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two-photon excitation spinning-disk confocal microscopy  (Kamada)
90
Kamada two-photon excitation spinning-disk confocal microscopy
Two Photon Excitation Spinning Disk Confocal Microscopy, supplied by Kamada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
two-photon excitation spinning-disk confocal microscopy - by Bioz Stars, 2026-03
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spinning disk confocal microscopy  (CEM Corporation)
90
CEM Corporation spinning disk confocal microscopy
Spinning Disk Confocal Microscopy, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
spinning disk confocal microscopy - by Bioz Stars, 2026-03
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spinning disk confocal microscopy wavefx  (Quorum Technologies)
90
Quorum Technologies spinning disk confocal microscopy wavefx
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Spinning Disk Confocal Microscopy Wavefx, supplied by Quorum Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
spinning disk confocal microscopy wavefx - by Bioz Stars, 2026-03
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inverted microscopes ix81with dsu spinning disk confocal microscopy system  (Evident Corporation)
90
Evident Corporation inverted microscopes ix81with dsu spinning disk confocal microscopy system
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Inverted Microscopes Ix81with Dsu Spinning Disk Confocal Microscopy System, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/inverted microscopes ix81with dsu spinning disk confocal microscopy system/product/Evident Corporation
Average 90 stars, based on 1 article reviews
inverted microscopes ix81with dsu spinning disk confocal microscopy system - by Bioz Stars, 2026-03
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spinning disk system for fast fluorescence confocal microscopy  (CrestOptics)
90
CrestOptics spinning disk system for fast fluorescence confocal microscopy
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Spinning Disk System For Fast Fluorescence Confocal Microscopy, supplied by CrestOptics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
spinning disk system for fast fluorescence confocal microscopy - by Bioz Stars, 2026-03
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spinning-disk confocal fluorescence microscopy  (Evident Corporation)
90
Evident Corporation spinning-disk confocal fluorescence microscopy
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Spinning Disk Confocal Fluorescence Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spinning-disk confocal fluorescence microscopy/product/Evident Corporation
Average 90 stars, based on 1 article reviews
spinning-disk confocal fluorescence microscopy - by Bioz Stars, 2026-03
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disk confocal microscopy  (Evident Corporation)
90
Evident Corporation disk confocal microscopy
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Disk Confocal Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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confocal microscope based on spinning disk confocal microscopy  (NanoFocus Inc)
90
NanoFocus Inc confocal microscope based on spinning disk confocal microscopy
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Confocal Microscope Based On Spinning Disk Confocal Microscopy, supplied by NanoFocus Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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confocal microscope based on spinning disk confocal microscopy - by Bioz Stars, 2026-03
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spinning-disk confocal microscopy  (Janssen)
90
Janssen spinning-disk confocal microscopy
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Spinning Disk Confocal Microscopy, supplied by Janssen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spinning-disk confocal microscopy/product/Janssen
Average 90 stars, based on 1 article reviews
spinning-disk confocal microscopy - by Bioz Stars, 2026-03
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spinning disk confocal microscopy  (Evident Corporation)
90
Evident Corporation spinning disk confocal microscopy
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Spinning Disk Confocal Microscopy, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spinning disk confocal microscopy/product/Evident Corporation
Average 90 stars, based on 1 article reviews
spinning disk confocal microscopy - by Bioz Stars, 2026-03
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laser spinning disk livecell confocal microscopy system  (universal imaging inc)
90
universal imaging inc laser spinning disk livecell confocal microscopy system
Western blot and <t>microscopy</t> analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.
Laser Spinning Disk Livecell Confocal Microscopy System, supplied by universal imaging inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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laser spinning disk livecell confocal microscopy system - by Bioz Stars, 2026-03
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Image Search Results


Western blot and microscopy analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.

Journal: The Journal of Biological Chemistry

Article Title: ERp57 Modulates STAT3 Signaling from the Lumen of the Endoplasmic Reticulum *

doi: 10.1074/jbc.M109.054015

Figure Lengend Snippet: Western blot and microscopy analysis of ERp57-deficient cells. A, Western blot analysis of wild-type, ERp57-deficient cells (ERp57−/−), and ERp57−/− cells expressing full-length recombinant ERp57 (ERp57−/−-ERp57ER) or cytoplasmic-targeted, no signal sequence ERp57 (ERp57−/−-ERp57cyt). Blots were probed with rabbit anti-ERp57 antibodies (lower protein band corresponds to a nonspecific immunoreactivity) and anti-GAPDH antibodies. In B, immunostaining of wild-type cells (wt), ERp57-deficient cells (ERp57−/−), ERp57-deficient cells expressing full-length, ER-targeted recombinant ERp57 (ERp57−/−-ERp57ER), ERp57-deficient cells expressing cytoplasmically targeted recombinant ERp57 (ERp57−/−-ERp57cyt), calreticulin-deficient cells (crt−/−), calreticulin-deficient cells expressing full-length calreticulin (crt−/−-CRT), calreticulin-deficient cells expressing loss of ERp57 binding mutant (crt−/−-CRTE239R) or ERp57 binding mutant of calreticulin (crt−/−-CRTG242A). Cells were stained with anti-ERp57 antibodies and Texas Red-conjugated concanavalin A. In each panel images represent staining with anti-ERp57 antibodies, ConA staining, phase-contrast, and merged images of the cells. Scale bar = 16 μm. C, Western blot analysis of cell lysate, cytoplasmic, and microsomal (containing ER) fractions of wild-type, ERp57-deficient (ERp57−/−), and ERp57-deficient cell lines expressing cytoplasmic targeted ERp57 (ERp57−/−-ERp57cyt). D, flow cytometry analysis of specific cell lines was carried out with anti-ERp57 antibodies. Wild-type, ERp57−/−-Erp57ER, ERp57−/−-Erp57CYS, crt−/−, crt−/−-CRT, crt−/−-CRTE239R, crt−/−-CRTG242A cell lines were used for the analysis. Results are presented as the relative mean florescence intensity after subtracting unspecific staining of ERp57−/− cells. M2 represents the gate set on cells stained with antibody. E, electron microscopy analysis of wild-type and ERp57-deficient cells. The arrows indicate the location of the ER. Scale bar = 0.2 μm. F, Western blot analysis of ER chaperone proteins and oxidoreductases in wild-type (wt) and ERp57-deficient (ERp57−/−) mouse embryonic fibroblasts. Antibodies used to probe the Western blot are indicated to the right of each panel. Lower protein band in the ERp57 lanes corresponds to a nonspecific immunoreactivity. CNX, calnexin; CRT, calreticulin.

Article Snippet: The coverslips were mounted onto glass slides and fluorescent signals visualized using spinning disk confocal microscopy (WaveFX from Quorum Technologies, Guelph, Canada) set up on an Olympus IX-81 inverted stand (Olympus, Markham, Canada).

Techniques: Western Blot, Microscopy, Expressing, Recombinant, Sequencing, Immunostaining, Binding Assay, Mutagenesis, Staining, Flow Cytometry, Electron Microscopy